PoplarV1, Main, Exploration, bibRecord, 003893

If the antibody fails--a mass western approach.

Identifieur interne : 003893 ( Main/Exploration ); précédent : 003892; suivant : 003894

If the antibody fails--a mass western approach.

Auteurs : Ute Lehmann [Allemagne] ; Stefanie Wienkoop ; Hendrik Tschoep ; Wolfram Weckwerth

Source :

The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2008.

RBID : pubmed:18485062

Descripteurs français

KwdFr :
- Arabidopsis (composition chimique), Arabidopsis (enzymologie), Basse température (MeSH), Chromatographie en phase liquide (méthodes), Feuilles de plante (composition chimique), Feuilles de plante (enzymologie), Glucosyltransferases (isolement et purification), Isoformes de protéines (MeSH), Masse moléculaire (MeSH), Protéines recombinantes (isolement et purification), Protéines végétales (composition chimique), Sensibilité et spécificité (MeSH), Spectrométrie de masse (méthodes), Électrophorèse sur gel de polyacrylamide (méthodes).
MESH :
- composition chimique : Arabidopsis, Feuilles de plante, Protéines végétales.
- enzymologie : Arabidopsis, Feuilles de plante.
- isolement et purification : Glucosyltransferases, Protéines recombinantes.
- méthodes : Chromatographie en phase liquide, Spectrométrie de masse, Électrophorèse sur gel de polyacrylamide.
- Basse température, Isoformes de protéines, Masse moléculaire, Sensibilité et spécificité.

English descriptors

KwdEn :
- Arabidopsis (chemistry), Arabidopsis (enzymology), Chromatography, Liquid (methods), Cold Temperature (MeSH), Electrophoresis, Polyacrylamide Gel (methods), Glucosyltransferases (isolation & purification), Mass Spectrometry (methods), Molecular Weight (MeSH), Plant Leaves (chemistry), Plant Leaves (enzymology), Plant Proteins (chemistry), Protein Isoforms (MeSH), Recombinant Proteins (isolation & purification), Sensitivity and Specificity (MeSH).
MESH :
- chemical , chemistry : Plant Proteins.
- chemical , isolation & purification : Glucosyltransferases, Recombinant Proteins.
- chemistry : Arabidopsis, Plant Leaves.
- enzymology : Arabidopsis, Plant Leaves.
- methods : Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry.
- Cold Temperature, Molecular Weight, Protein Isoforms, Sensitivity and Specificity.

Abstract

SUMMARY

Sucrose-phosphate synthase (SPS) has attracted the interest of plant scientists for decades. It is the key enzyme in sucrose metabolism and is under investigation in various plant species, e.g. spinach, tobacco, poplar, resurrection plants, maize, rice, kiwi and Arabidopsis thaliana. In A. thaliana, there are four distinct SPS isoforms. Their expression is thought to depend on environmental conditions and plant tissue. However, these data were derived from mRNA expression levels only. No data on SPS protein identification from crude extracts have been available until now. An antibody approach failed to distinguish the four isoforms. Therefore, we developed a method for SPS quantification and isoform-specific identification in A. thaliana complex protein samples. Samples were separated on SDS-PAGE, digested and directly applied to liquid chromatography/triple-stage quadrupole mass spectrometry (LC/TSQ-MS). In this approach, known as mass Western, samples were analysed in multi-reaction monitoring (MRM) mode, so that all four SPS isoforms could be measured in one experiment. In addition to the relative quantification, stable isotope-labelled internal peptide standards allowed absolute quantification of SPS proteins. Protein extracts from various plant tissues, samples harvested during the day or the night, and cold-stressed plants were analysed. The stress-specific SPS5a isoform showed increased concentrations in cold-stressed leaf material.

DOI: 10.1111/j.1365-313X.2008.03554.x
PubMed: 18485062
PubMed Central: PMC2607522

Affiliations:

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Le document en format XML

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<ReferenceList><Reference><Citation>Plant J. 2002 May;30(4):489-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12028578</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 1992 Sep;100(1):502-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16652990</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 1998 Jun;1(3):230-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10066585</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2001 Jul;13(7):1499-510</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11449047</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2000 Dec 15;290(5499):2110-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11118138</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Evol. 2002 Mar;54(3):322-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11847558</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1978 Sep 10;253(17):5952-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">210163</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2001 Sep;127(1):252-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11553753</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2006;7(8):R76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16916443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1999 Apr;11(4):707-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10213788</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2007;8:216</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17587460</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2006;57(7):1529-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16574747</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2000 Oct;124(2):641-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11027714</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2005 Oct;4(10):1614-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16030010</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1990 Aug;2(8):755-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2152125</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2006 Sep;47(9):1309-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16936336</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2001 Nov;52(364):2169-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11604456</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2002 Apr;53(370):959-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11912238</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Comp Biochem Physiol B Biochem Mol Biol. 2001 Aug;130(1):105-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11470449</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1998 May;10(5):791-800</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9596637</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2000 Apr;122(4):1137-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10759509</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1997 Jan;9(1):5-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9014361</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Biochem Biophys. 1981 Nov;212(1):237-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6272652</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2001 Jan;125(1):488-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11154356</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Expert Rev Proteomics. 2006 Dec;3(6):597-610</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17181474</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2003 Jun;6(3):208-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12753969</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem J. 2001 Feb 15;354(Pt 1):67-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11171080</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Electrophoresis. 1996 Oct;17(10):1643-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8957197</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2002 Apr;30(2):221-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12000458</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6940-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12771378</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2004 Dec;16(12):3304-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15548738</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2005 Nov;139(3):1163-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16244140</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2001 May;26(4):421-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11439129</ArticleId>
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<Reference><Citation>J Exp Bot. 2001 Dec;52(365):2301-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11709580</ArticleId>
</ArticleIdList>
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<Reference><Citation>Planta. 1997;202(1):106-11</Citation>
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<country name="Allemagne"><region name="Brandebourg"><name sortKey="Lehmann, Ute" sort="Lehmann, Ute" uniqKey="Lehmann U" first="Ute" last="Lehmann">Ute Lehmann</name>
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   |texte=   If the antibody fails--a mass western approach.
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If the antibody fails--a mass western approach.

If the antibody fails--a mass western approach.

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